Lowenstein-Jensen is a medium enriched with egg, potato, or serum commonly used to cultivate mycobacterium.
Members of the genus Mycobacterium act as the causative agents for tuberculosis and leprosy. Mycobacterium contain an abundance of fats and waxes in their cell walls, including mycolic acid.
Acid-Fast Technique — Mixed
Positive (+) result— Acid-fast organisms will be red because carbolfuchsin stain will adhere to acid-fast bacterium.
carbolfuchsin — the primary dye.
acid-alcohol — decolorizer.
methylene blue — the secondary stain.
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There are over 70 species in the genus Mycobacterium, many of which cause disease in humans. Hansen identified Mycobacterium leprae as the first species of the genus Mycobacterium in 1874. Furthermore, in 1882, Robert Koch isolated the mammalian tubercle bacillus and established its role as a causative agent of tuberculosis, as it met all of Koch's postulates. Mycobacterium tuberculosis was later identified as the causative agent of tuberculosis. https://labweeks.com/mycobacterium/
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